Profiles of Antibody Responses against Severe Acute Respiratory Syndrome Coronavirus Recombinant Proteins and Their Potential Use as Diagnostic Markers
Identifieur interne : 005186 ( Main/Exploration ); précédent : 005185; suivant : 005187Profiles of Antibody Responses against Severe Acute Respiratory Syndrome Coronavirus Recombinant Proteins and Their Potential Use as Diagnostic Markers
Auteurs : Yee-Joo Tan ; Phuay-Yee Goh ; Burtram C. Fielding ; Shuo Shen ; Chih-Fong Chou ; Jian-Lin Fu ; Hoe Nam Leong ; Yee Sin Leo ; Eng Eong Ooi ; Ai Ee Ling ; Seng Gee Lim ; Wanjin HongSource :
- Clinical and Diagnostic Laboratory Immunology [ 1071-412X ] ; 2004.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (sang), Antigènes viraux (génétique), Antigènes viraux (immunologie), Cellules cultivées, Humains, Immunoglobuline A (sang), Immunoglobuline G (sang), Immunoglobuline M (sang), Marqueurs biologiques, Plasmides, Sensibilité et spécificité, Spécificité des anticorps, Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (immunologie), Technique d'immunofluorescence, Virus du SRAS (génétique), Virus du SRAS (immunologie), Virus du SRAS (isolement et purification).
- MESH :
- diagnostic : Syndrome respiratoire aigu sévère.
- génétique : Antigènes viraux, Virus du SRAS.
- immunologie : Antigènes viraux, Syndrome respiratoire aigu sévère, Virus du SRAS.
- isolement et purification : Virus du SRAS.
- sang : Anticorps antiviraux, Immunoglobuline A, Immunoglobuline G, Immunoglobuline M.
- Animaux, Cellules cultivées, Humains, Marqueurs biologiques, Plasmides, Sensibilité et spécificité, Spécificité des anticorps, Technique d'immunofluorescence.
English descriptors
- KwdEn :
- Animals, Antibodies, Viral (blood), Antibody Specificity, Antigens, Viral (genetics), Antigens, Viral (immunology), Biomarkers, Cells, Cultured, Fluorescent Antibody Technique, Humans, Immunoglobulin A (blood), Immunoglobulin G (blood), Immunoglobulin M (blood), Plasmids, SARS Virus (genetics), SARS Virus (immunology), SARS Virus (isolation & purification), Sensitivity and Specificity, Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (immunology).
- MESH :
- chemical , blood : Antibodies, Viral, Immunoglobulin A, Immunoglobulin G, Immunoglobulin M.
- chemical , genetics : Antigens, Viral.
- chemical , immunology : Antigens, Viral.
- diagnosis : Severe Acute Respiratory Syndrome.
- genetics : SARS Virus.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- isolation & purification : SARS Virus.
- Animals, Antibody Specificity, Biomarkers, Cells, Cultured, Fluorescent Antibody Technique, Humans, Plasmids, Sensitivity and Specificity.
Abstract
A new coronavirus (severe acute respiratory syndrome coronavirus [SARS-CoV]) has been identified to be the etiological agent of severe acute respiratory syndrome. Given the highly contagious and acute nature of the disease, there is an urgent need for the development of diagnostic assays that can detect SARS-CoV infection. For determination of which of the viral proteins encoded by the SARS-CoV genome may be exploited as diagnostic antigens for serological assays, the viral proteins were expressed individually in mammalian and/or bacterial cells and tested for reactivity with sera from SARS-CoV-infected patients by Western blot analysis. A total of 81 sera, including 67 from convalescent patients and seven pairs from two time points of infection, were analyzed, and all showed immunoreactivity towards the nucleocapsid protein (N). Sera from some of the patients also showed immunoreactivity to U274 (59 of 81 [73%]), a protein that is unique to SARS-CoV. In addition, all of the convalescent-phase sera showed immunoreactivity to the spike (S) protein when analyzed by an immunofluorescence method utilizing mammalian cells stably expressing S. However, samples from the acute phase (2 to 9 days after the onset of illness) did not react with S, suggesting that antibodies to N may appear earlier than antibodies to S. Alternatively, this could be due to the difference in the sensitivities of the two methods. The immunoreactivities to these recombinant viral proteins are highly specific, as sera from 100 healthy donors did not react with any of them. These results suggest that recombinant N, S, and U274 proteins may be used as antigens for the development of serological assays for SARS-CoV.
Url:
DOI: 10.1128/CDLI.11.2.362-371.2004
PubMed: 15013989
PubMed Central: 371215
Affiliations:
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Le document en format XML
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Fielding</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Shen, Shuo" sort="Shen, Shuo" uniqKey="Shen S" first="Shuo" last="Shen">Shuo Shen</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Chou, Chih Fong" sort="Chou, Chih Fong" uniqKey="Chou C" first="Chih-Fong" last="Chou">Chih-Fong Chou</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Fu, Jian Lin" sort="Fu, Jian Lin" uniqKey="Fu J" first="Jian-Lin" last="Fu">Jian-Lin Fu</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Nam Leong, Hoe" sort="Nam Leong, Hoe" uniqKey="Nam Leong H" first="Hoe" last="Nam Leong">Hoe Nam Leong</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Sin Leo, Yee" sort="Sin Leo, Yee" uniqKey="Sin Leo Y" first="Yee" last="Sin Leo">Yee Sin Leo</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Eong Ooi, Eng" sort="Eong Ooi, Eng" uniqKey="Eong Ooi E" first="Eng" last="Eong Ooi">Eng Eong Ooi</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Ee Ling, Ai" sort="Ee Ling, Ai" uniqKey="Ee Ling A" first="Ai" last="Ee Ling">Ai Ee Ling</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Gee Lim, Seng" sort="Gee Lim, Seng" uniqKey="Gee Lim S" first="Seng" last="Gee Lim">Seng Gee Lim</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Hong, Wanjin" sort="Hong, Wanjin" uniqKey="Hong W" first="Wanjin" last="Hong">Wanjin Hong</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">Clinical and Diagnostic Laboratory Immunology</title><idno type="ISSN">1071-412X</idno><idno type="eISSN">1098-6588</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Viral (blood)</term><term>Antibody Specificity</term><term>Antigens, Viral (genetics)</term><term>Antigens, Viral (immunology)</term><term>Biomarkers</term><term>Cells, Cultured</term><term>Fluorescent Antibody Technique</term><term>Humans</term><term>Immunoglobulin A (blood)</term><term>Immunoglobulin G (blood)</term><term>Immunoglobulin M (blood)</term><term>Plasmids</term><term>SARS Virus (genetics)</term><term>SARS Virus (immunology)</term><term>SARS Virus (isolation & purification)</term><term>Sensitivity and Specificity</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term><term>Severe Acute Respiratory Syndrome (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (sang)</term><term>Antigènes viraux (génétique)</term><term>Antigènes viraux (immunologie)</term><term>Cellules cultivées</term><term>Humains</term><term>Immunoglobuline A (sang)</term><term>Immunoglobuline G (sang)</term><term>Immunoglobuline M (sang)</term><term>Marqueurs biologiques</term><term>Plasmides</term><term>Sensibilité et spécificité</term><term>Spécificité des anticorps</term><term>Syndrome respiratoire aigu sévère (diagnostic)</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Technique d'immunofluorescence</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (immunologie)</term><term>Virus du SRAS (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term><term>Immunoglobulin A</term><term>Immunoglobulin G</term><term>Immunoglobulin M</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antigens, Viral</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Antigènes viraux</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Antigènes viraux</term><term>Syndrome respiratoire aigu sévère</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term><term>Immunoglobuline A</term><term>Immunoglobuline G</term><term>Immunoglobuline M</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Antibody Specificity</term><term>Biomarkers</term><term>Cells, Cultured</term><term>Fluorescent Antibody Technique</term><term>Humans</term><term>Plasmids</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules cultivées</term><term>Humains</term><term>Marqueurs biologiques</term><term>Plasmides</term><term>Sensibilité et spécificité</term><term>Spécificité des anticorps</term><term>Technique d'immunofluorescence</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>A new coronavirus (severe acute respiratory syndrome coronavirus [SARS-CoV]) has been identified to be the etiological agent of severe acute respiratory syndrome. Given the highly contagious and acute nature of the disease, there is an urgent need for the development of diagnostic assays that can detect SARS-CoV infection. For determination of which of the viral proteins encoded by the SARS-CoV genome may be exploited as diagnostic antigens for serological assays, the viral proteins were expressed individually in mammalian and/or bacterial cells and tested for reactivity with sera from SARS-CoV-infected patients by Western blot analysis. A total of 81 sera, including 67 from convalescent patients and seven pairs from two time points of infection, were analyzed, and all showed immunoreactivity towards the nucleocapsid protein (N). Sera from some of the patients also showed immunoreactivity to U274 (59 of 81 [73%]), a protein that is unique to SARS-CoV. In addition, all of the convalescent-phase sera showed immunoreactivity to the spike (S) protein when analyzed by an immunofluorescence method utilizing mammalian cells stably expressing S. However, samples from the acute phase (2 to 9 days after the onset of illness) did not react with S, suggesting that antibodies to N may appear earlier than antibodies to S. Alternatively, this could be due to the difference in the sensitivities of the two methods. The immunoreactivities to these recombinant viral proteins are highly specific, as sera from 100 healthy donors did not react with any of them. These results suggest that recombinant N, S, and U274 proteins may be used as antigens for the development of serological assays for SARS-CoV.</p></div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Chou, Chih Fong" sort="Chou, Chih Fong" uniqKey="Chou C" first="Chih-Fong" last="Chou">Chih-Fong Chou</name><name sortKey="Ee Ling, Ai" sort="Ee Ling, Ai" uniqKey="Ee Ling A" first="Ai" last="Ee Ling">Ai Ee Ling</name><name sortKey="Eong Ooi, Eng" sort="Eong Ooi, Eng" uniqKey="Eong Ooi E" first="Eng" last="Eong Ooi">Eng Eong Ooi</name><name sortKey="Fielding, Burtram C" sort="Fielding, Burtram C" uniqKey="Fielding B" first="Burtram C." last="Fielding">Burtram C. Fielding</name><name sortKey="Fu, Jian Lin" sort="Fu, Jian Lin" uniqKey="Fu J" first="Jian-Lin" last="Fu">Jian-Lin Fu</name><name sortKey="Gee Lim, Seng" sort="Gee Lim, Seng" uniqKey="Gee Lim S" first="Seng" last="Gee Lim">Seng Gee Lim</name><name sortKey="Goh, Phuay Yee" sort="Goh, Phuay Yee" uniqKey="Goh P" first="Phuay-Yee" last="Goh">Phuay-Yee Goh</name><name sortKey="Hong, Wanjin" sort="Hong, Wanjin" uniqKey="Hong W" first="Wanjin" last="Hong">Wanjin Hong</name><name sortKey="Nam Leong, Hoe" sort="Nam Leong, Hoe" uniqKey="Nam Leong H" first="Hoe" last="Nam Leong">Hoe Nam Leong</name><name sortKey="Shen, Shuo" sort="Shen, Shuo" uniqKey="Shen S" first="Shuo" last="Shen">Shuo Shen</name><name sortKey="Sin Leo, Yee" sort="Sin Leo, Yee" uniqKey="Sin Leo Y" first="Yee" last="Sin Leo">Yee Sin Leo</name><name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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